feeder free growth of undifferentiated human embryonic stem cells

feeder free growth of undifferentiated human embryonic stem cells

C Xu M K Carpenter. Get PDF. References 15 Citations Nature Genetics. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture opens in new tab.

Developmental Biology. M Amit J A Thomson. Integrins: versatility, modulation, and signaling in cell adhesion opens in new tab. R O Hynes. Variants of the alpha 6 beta 1 laminin receptor in early murine development: distribution, molecular cloning and chromosomal localization of the mouse integrin alpha 6 subunit opens in new tab.

Cell Adhesion and Communication. B P Hierck A Sonnenberg. Specific association of human telomerase activity with immortal cells and cancer opens in new tab. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19 : — Human feeder layers for human embryonic stem cells.

Biol Reprod 68 : — Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 70 : — Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn : — Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20 : — Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture.

Stem Cells 21 : — Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. Comparative biology of the IGF system in endometrium, decidua, and placenta, and clinical implications for foetal growth and implantation disorders.

Placenta 24 : — Giudice LC. Genes associated with embryonic attachment and implantation and the role of progesterone. J Reprod Med 44 :suppl 2 — Defining the actions of transforming growth factor beta in reproduction.

Bioessays 24 : — Tabibzadeh S. Homeostasis of extracellular matrix by TGF-beta and lefty. Front Biosci 7 : — The expression of Smads in human endometrium and regulation and induction in endometrial epithelial and stromal cells by transforming growth factor-beta. J Clin Endocrinol Metab 88 : — Biology and action of colony-stimulating factor Mol Reprod Dev 46 : 4 — J Assist Reprod Genet 13 : — The role of leukemia inhibitory factor and interleukin-6 in human reproduction.

Hum Reprod suppl 3 — Leukemia inhibitory factor in human reproduction. Fertil Steril 76 : — Interleukin-1 system crosstalk between embryo and endometrium in implantation. Hum Reprod suppl 2 43 — Role of endometrial factors in regulating secretion of components of the immunoreactive human embryonic interleukin-1 system during embryonic development. Biol Reprod 54 : — Williams, R. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells.

Kleinman, H. Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21 , — Bisell, D. Support of cultured hepatocytes by a laminin-rich gel. Bodnar, A. Extension of life-span by introduction of telomerase into normal human cells. Cooper, A. Subunits of laminin are differentially synthesized in mouse eggs and early embryos. Ekblom, P. Cell—matrix interactions and cell adhesion during development.

Cell Biol. Show 5 more references 10 of Smart citations by scite. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles. Explore citation contexts and check if this article has been supported or disputed. Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings.

Improving single-cell transcriptome sequencing efficiency with a microfluidic phase-switch device. Developments in cell culture systems for human pluripotent stem cells. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells.

Adaptation of human embryonic stem cells to feeder-free and matrix-free culture conditions directly on plastic surfaces. Autogeneic feeders for the culture of undifferentiated human embryonic stem cells in feeder and feeder-free conditions. Characterization and differentiation of human embryonic stem cells. Advertisement Hide.

Protocol First Online: 30 July This is a preview of subscription content, log in to check access. Thomson J. Jones J. Bibliographic Citation Nature Biotechnology October; 19 10 : Permanent Link Find in a Library.

Human embryonic stem hjman cells are usually established and maintained on mouse embryonic fibroblast MEFs feeder layers. In this study, we attempted undifderentiated establish new hES cell lines using human uterine endometrial cells feeder free growth of undifferentiated human embryonic stem cells to prevent the risks associated with animal feeder free growth of undifferentiated human embryonic stem cells cells and for their eventual application in cell-replacement therapy. Inner cell masses ICMs of cultured blastocysts were isolated by wmbryonic and then cultured on mitotically inactivated hUEC feeder layers. Cultured ICMs formed colonies by continuous proliferation and were allowed to proliferate continuously for 40, 50, and 55 passages. The established hES cell lines Miz-hES,and -9, respectively exhibited typical hES cells characteristics, including continuous growth, expression of specific markers, normal karyotypes, and differentiation capacity. The hUEC feeders have the advantage that they can be used for many passages, whereas MEF feeder cells can only be used as feeder cells for a limited number of passages. The hUECs are available to establish and maintain hES cells, and the high expression of embryotrophic factors and extracellular matrices by hUECs may be important to the efficient growth of hES cells. Clinical applications require the establishment and expansion of hES cells under stable xeno-free culture systems. Human embryonic stem hES cells were first isolated from inner cell masses ICMs of human blastocysts by Thomson and colleagues [ 1 ]. The hES cells exhibit unique features, including continuous growth, a high level of telomerase activity, normal karyotypes, and differentiation capacity in vivo and in vitro. They express pluripotent cell-specific markers: feeder free growth of undifferentiated human embryonic stem cells positive for stage-specific embryonic antigen SSEA -3 and -4, tumor rejection antigen TRA andand a high level of vlc media for mac free download phosphatase APase activity, but are negative for SSEA-1 [ 1 — 7 ]. Feeder free growth of undifferentiated human embryonic stem cells also express Oct-4, a transcription factor that is specific to ICMs of blastocysts [ undifferentiaetd5 — 8 ]. In contrast with the culturing of mES cells, feeder cell layers and basic fibroblast growth factor bFGF are necessary to maintain hES cells in an undifferentiated state with or without LIF, but LIF alone cannot support the continuous humqn of hES cells with growtth the differentiation [ 13 embryoinc, 9 ]. The hES cells are usually embgyonic and maintained on mouse embryonic fibroblast MEF feeder layers. Because animal feeder cells are associated with risks, including pathogen transmission and viral infection, and ethical problems, many stem cell researchers have investigated different culture conditions for hES cells, such as a feeder-free culture system. However, this culture system also requires the feeder free growth of undifferentiated human embryonic stem cells and continuous culturing of MEF cells to obtain MEF-CM medium, and ste possibilities of mouse retroviral infections and pathogen transmission remain [ 11 — 13 ]. Attempts to prevent infections and pathogen transmission have involved the cultivation of hES cells embryoic human feeder layers, such as fetal muscle, fetal skin, adult fallopian unxifferentiated feeder free growth of undifferentiated human embryonic stem cells cells [ 14 ], foreskin fibroblasts [ 611 ], and adult feeder free growth of undifferentiated human embryonic stem cells cells [ 15 ]. Additionally, Richards and colleagues [ 16 ] reported the testing of various human feeder cells derived from fetal muscle, skinadult lung, skin, fallopian tube, muscle, endometriumand neonatal foreskin tissues, and some of these cells are now available for use. feeder free growth of undifferentiated human embryonic stem cells Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF​). PDF | Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse. Conventionally, human embryonic stem cells (hESCs) are cultured on feeder cells. The most commonly used feeder cells are mouse embryonic. Feeder-free growth of undifferentiated human embryonic stem cells. Nature Biotechnology. C XuM K Carpenter. Get PDF. Share. were the first to report the development of a feeder-free culture system for the growth of human ES cells. Growth of Undifferentiated Human Embryonic Stem Cells. Petra Stojkovic,a Majlinda 13], not all human feeders and cell-free matrices support the culture of​. Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells. Creator. Xu, Chunhui. Inokuma, Margaret S. Denham, Jerrod. Golds, Kathaleen. Kundu. Human embryonic stem (hES) cells are usually established and maintained on mouse Feeder-free growth of undifferentiated human embryonic stem cells. The exposure of ES cells to these components has been a major concern for the potential therapeutic use of ES cells because of their immunogenicity and potential pathogenicity. Back to top. J Cell Sci. Click here for file 6. Nat Genet. Recent progress has been made in studies using human ES cells, in which these cells could be successfully maintained in a proliferative and pluripotent state even when the feeder cells and serum were replaced with mostly defined proteins or chemicals [ Ludwig et al. Additional file 10 Fig. Stem Cell Rev 26, — Among all the newly derived human ES cell lines, twelve lines have gained the most attention. Published online Oct Although animal-derived serum was later substituted by synthetic serum [ Goldsborough et al. At less than this density, the ES cells did not form cell masses and died soon after seeding, suggesting that the formation of a certain size of cell mass caused by PVA-induced expression of adhesive molecules is a key for the proliferation of undifferentiated ES cells in suspension cultures. Supplementary Material Supp 1 Click here to view. feeder free growth of undifferentiated human embryonic stem cells